Scientific supervisor
| Name | Ewa Stępień |
| Email: | e.stepien@uj.edu.pl |
| Department | Department of Medical Physics |
| Laboratory | Centrum Teranostyki |
| Group webpage | https://zfm.if.uj.edu.pl/projekty |
Short description
In the laboratory, cell cultures of both primary cell lines (endothelial cells) and immortalized cell lines (human melanoma cells) are conducted.
The practice plan includes the following steps:
- Familiarization with the theoretical basics of performing cell cultures.
- Understanding the principles of working with biological material under sterile conditions.
- Learning techniques for cell line preparation.
- Conducting experiments and measurements based on the specific project:
- Cell viability tests.
- Differential centrifugation.
- Characterization of specific antigens on cell surfaces.
- Microscopic observations.
- 3D cell culture (spheroids).
At the end of the practice, students compile a report summarizing their measurements.
Main research tools
The laboratory is equipped with the following devices:
- Imaging flow cytometer Celligo
- CO2 incubator with automatic temperature and CO2 concentration control (ICO150med)
- Laminar flow hood (MSC-Advantage)
- Centrifuge (Z300K; Hermle)
- Inverted laboratory microscope
- Automated cell counter (LUNA II)
- 3D cell culture reactor (ClinoStar)
Additional requirements to the candidate
Students of biophysics, biotechnology, medical chemistry, biology, pharmacy, and medical analytics
Possibility to continue student internship in the form of:
- Diploma thesis (master's or bachelor's degree): Yes
- PhD study: Yes