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Flow cytometry of egzosomes and extracellular vesicles.

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Scientific supervisor

Name Ewa Stępień
Email: e.stepien@uj.edu.pl
Department Department of Medical Physics
Laboratory Laboratory of cytometry and spectroscopy of nano-objects
Group webpage http://www.zfm.if.uj.edu.pl/en_GB/grupy-badawcze/laboratorium-cytometrii-i-spektroskopii-nanoobiektow

Short description

The laboratory measures exosome and extracellular vesicle (EV) samples from cell cultures and body fluids by fluorescence flow cytometry and tunable pulse conductometry.

The laboratory is equipped with the following devices:

  • ImageStream Luminex flow cytometer
  • Sorvall ultracentrifuge with angular rotor; Micro-Ultracentrifuge Sorvall mX150 +; Thermo Scientific
  • Izon particle size analyzer (qNano)
  • Izon cell size analyzer (qMikro)

The internship plan provides for:

  1. Getting to know the theoretical basics of performing cytometric and flow resistance measurements,
  2. Getting to know the rules of working with biological and clinical material
  3. Getting to know the techniques of exosome preparation by filtration and ultracentrifugation methods
  4. Performing experiments and measurements depending on the project being carried out:
    • Concentration of urine samples or culture medium by filtration
    • Differential centrifugation
    • Ultracentrifugation
    • Determination of the number of exosomes in a sample
    • Characteristics of exosome-specific antigens
    • Evaluation of the extracellular vesicles size distribution

At the end of the internship, the student prepares a report on the measurements taken.

Main research tools

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Additional requirements to the candidate

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Possibility to continue student internship in the form of:

  • Diploma thesis (master's or bachelor's degree): Yes
  • PhD study: Yes